A Competitive Index Assay Identifies Several Ralstonia solanacearum Type III Effector Mutant Strains with Reduced Fitness in Host Plants
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چکیده
منابع مشابه
Ralstonia solanacearum requires F-box-like domain-containing type III effectors to promote disease on several host plants.
The phytopathogenic bacterium Ralstonia solanacearum encodes a family of seven type III secretion system (T3SS) effectors that contain both a leucine-rich repeat and an F-box domain. This structure is reminiscent of a class of typical eukaryotic proteins called F-box proteins. The latter, together with Skp1 and Cullin1 subunits, constitute the SCF-type E3 ubiquitin ligase complex and control sp...
متن کاملCurrent knowledge on the Ralstonia solanacearum type III secretion system
Ralstonia solanacearum was ranked in a recent survey the second most important bacterial plant pathogen, following the widely used research model Pseudomonas syringae (Mansfield et al., 2012). The main reason is that bacterial wilt caused by R. solanacearum is the world’s most devastating bacterial plant disease (http://faostat. fao.org), threatening food safety in tropical and subtropical agri...
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A fluorogenic (TaqMan) PCR assay was developed to detect Ralstonia solanacearum strains. Two fluorogenic probes were utilized in a multiplex reaction; one broad-range probe (RS) detected all biovars of R. solanacearum, and a second more specific probe (B2) detected only biovar 2A. Amplification of the target was measured by the 5' nuclease activity of Taq DNA polymerase on each probe, resulting...
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Banana wilt outbreaks that are attributable to Moko disease-causing strains of the pathogen Ralstonia solanacearum (Rs) remain a social and economic burden for both multinational corporations and subsistence farmers. All known Moko strains belong to the phylotype II lineage, which has been previously recognized for its broad genetic basis. Moko strains are paraphyletic and are distributed among...
متن کاملA Ralstonia solanacearum Type III Effector Directs the Production of the Plant Signal Metabolite Trehalose-6-Phosphate
UNLABELLED The plant pathogen Ralstonia solanacearum possesses two genes encoding a trehalose-6-phosphate synthase (TPS), an enzyme of the trehalose biosynthetic pathway. One of these genes, named ripTPS, was found to encode a protein with an additional N-terminal domain which directs its translocation into host plant cells through the type 3 secretion system. RipTPS is a conserved effector in ...
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ژورنال
عنوان ژورنال: Molecular Plant-Microbe Interactions®
سال: 2010
ISSN: 0894-0282,1943-7706
DOI: 10.1094/mpmi-23-9-1197